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Genomic technologies  

Laboratory of genomic technologies

 

For several years our laboratory has been devoted to apply genomic technologies to study human diseases and cellular models using multidisciplinary approaches such as biochemistry, biology, biotechnology and bioinformatics. In particular, we have gained a lot of experience in the study of gene expression profiles in various biological samples (cell lines and clinical tissues) using microarray technology (home-made microarray and Affymetrix platform). In parallel, the group has been involved in the studying the genome variation (analysis of single nucleotide polymorphisms SNPs and copy number analysis) present in DNA samples obtained from human pathological specimens. Thanks to the presence of a multidisciplinary team and our strong relationships with groups of bioinformatics, we have developed bioinformatics methodologies to analyze and to integrate complex biological data.

 

Main research lines

  1. Study of human tumors (renal cell carcinoma, melanoma) and models of cell lines (ATCC lines and primary cells) using genome-wide technologies to analyze genes and microRNA (gene expression profiling, microarrays)

  2. Study of rare diseases (Congenital hyperinsulinism of Infancy) using genomics technologies such as microarray (SNP array) and deep sequencing (next generation sequencing).

  3.  Development of methodologies for the integration of genomic data

  4. Development of analytical methods applied to nucleic acids (DNA, RNA and microRNA) based on high-throughput platforms (Collaboration with ITB-CNR).

 

Grants

Progetto CARIPLO: Biological effects and human health impacts of ultrafine particles source
Leader: Università Milano Bicocca
Partner Unit: ENEA e Università degli studi di Milano
Time: two years project (from 1/04/2014 to 31/03/2016)

 

Main instrumentation available to the group

Agilent Bionalyser 2100 (Agilent)
Nanodrop, (Celbio)
Stazione fluidica FS 250 (Affymetrix)
Scanner for GennChip x (Affymetrix)
Scanarray 5000 for microarray analysis (Perkin elmer)
Real time iCycler (Biorad)
Bioplex (Biorad)
Sequencer GAIIX and Myseq (Illumina)

 

Group composition

The Research Group is composed by Dr. Cristina Battaglia (Assistant Professor of Biochemistry, group leader), Maria Carla Proverbio (biologist), three PhD students (Cristina Cosentino, Luca Petiti, Simone Puccio). Collaboration with Genomic Unit of ITB-CNR, Segrate: Gianluca De Bellis, Ingrid Cifola, Eleonora Mangano (http://www.itb.cnr.it/web/genomics/research).

 

Contacts

Dott.ssa Cristina Battaglia  (E-mail. cristina.battaglia@unimi.it)
Skype: cristina.battaglia ; Phone : +390250330421
Location : L.I.T.A palace ,via F. Cervi 93, 20090 Segrate (MI), Italy, 3rd floor.

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